Optimization of Ultrasound-Assisted Extraction of Free Phenolic Compounds and in vitro Biological Activity from peach fruit Using Response Surface Methodology

In this study, the ultrasonic extraction (UAE) of free phenolic compounds and relative 18 biological activities of the Bulgarian peach variety 'Filina' was optimized using chemometric tech-19 niques (response surface methodology). A Box–Behnken design was used to reveal the variation of 20 hydro module, temperature, duration, and extractant on the total phenolic content, total flavonoid 21 content, antioxidant potential, and inhibitory activity on each yield. The results revealed that opti-22 mal conditions included a hydro module of 20, a duration of 39.328 minutes, a temperature of 70 23 °C, and an extractant of 96.638% to retrieve the highest level of bioactive compounds. The calculated 24 parameters were discovered to be following the projected values.